Axial localization with modulated-illumination extended-depth-of-field microscopy
نویسندگان
چکیده
منابع مشابه
Extended Depth of Field for Fluorescence Microscopy
High numerical aperture objectives result in extremely shallow depths of field, which may or may not be desired by the user of the microscope. When looking at extended objects, planes that arent necessarily perpendicular to the light path, or fluorophores that move in and out of focus, an extended depth of field (EDOF) would be useful to fully visualize the sample. PSF engineering can be applie...
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ژورنال
عنوان ژورنال: Biomedical Optics Express
سال: 2018
ISSN: 2156-7085,2156-7085
DOI: 10.1364/boe.9.001771